Rapid Molecular Detection of Tuberculosis
نویسنده
چکیده
n engl j med 364;2 nejm.org january 13, 2011 182 stem cells is the standard by which their pluripotent potential is evaluated.1-3 We believe that the intended application of human induced pluripotent stem-cell lines should determine the evaluation method. The issue of pluripotency becomes important for studies of the functional mechanism of reprogramming. However, a reproducible and rapid method to determine the quality of newly established human pluripotent stem cells is urgently required. Perhaps an epigenetic and gene-expression signature that selectively defines fully reprogrammed pluripotent stem cells can be identified, since the variation of teratoma results brings up the question of this assay’s value as a standard for proving pluripotency of human stem cells.4 We tested our lines for the reactivation of endogenous pluripotency genes, the silencing of retroviral transgenes, and the capacity to form the three germ layers. In close collaboration with the pathology department, we are in the process of correlating the results of teratoma assays with the ability to generate functional cardiac myocytes. Although induced cardiomyocytes may replace pluripotent stem cells for disease modeling, this technique needs to be reproducibly established in human cells. Alessandra Moretti, Ph.D. Jason T. Lam, Ph.D. Karl-Ludwig Laugwitz, M.D.
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